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Image Search Results
Journal: Molecular Pharmacology
Article Title: Discovery and Characterization of Novel GPR39 Agonists Allosterically Modulated by Zinc
doi: 10.1124/mol.116.106112
Figure Lengend Snippet: Concentration-dependent and GPR39-dependent cAMP (Gs) responses, as measured by the Glosensor assay, to GPR39-C3 (A), LY2784544 (B), or GSK2636771 (C) with various concentrations of Zn2+. Responses in the absence of Zn2+ are shown in (D), (E), and (F). Responses to ZnCl2, NiCl2, and NiSO4 are shown in (G) and (H) and (I), respectively. The results are the mean ± S.E.M. of at least three independent experiments performed in quadruplicate.
Article Snippet: Receptor-mediated Gs pathway signaling was measured using a split-luciferase receptor assay (
Techniques: Concentration Assay
Journal: Molecular Pharmacology
Article Title: Discovery and Characterization of Novel GPR39 Agonists Allosterically Modulated by Zinc
doi: 10.1124/mol.116.106112
Figure Lengend Snippet: Concentration-dependent cAMP production (Gs pathway) to GPR39-C3 (A), LY2784544 (B), and GSK2636771 (C) in the presence and absence of 100 μM Zn2+ at wild-type (WT) and H17A/H19A mutant receptors as measured by the Glosensor assay. Concentration-dependent cAMP production (Gs pathway) to ZnCl2 (D), NiCl2 (E), or NiSO4 (F) at wild-type or H17A/H19A mutant receptors as measured by the Glosensor assay. The results are the mean ± S.E.M. of at least eight independent experiments, each in quadruplicate.
Article Snippet: Receptor-mediated Gs pathway signaling was measured using a split-luciferase receptor assay (
Techniques: Concentration Assay, Mutagenesis